AMAZON™ SPEED ETD

The new amaZon speed ETD incorporates key inventions for unprecedented proteomics capabilities. The high dynamic range and complexity of proteomics samples due to post translational modifications require maximum speed and sensitivity to capture as many peptides as possible. The amaZon speed ion ETD trap sets new standards in protein identification due to its enhanced MS/MS speed, new SWIFT isolation and SMART fragmentation. This results in routine identification of more than 2500 unambiguously identified proteins in a single LC-MS/MS run from 5 µg of a HeLa lysate. The dual ion funnel transfer line is delivering absolute benchmark sensitivity. TECHNICAL DETAILS: • Bottom-up proteomics identifying more low abundant proteins due to greatly increased MS/MS duty cycle • Top-down sequencing (TDS) for highest protein sequence coverage • Highest confidence through market leading mass accuracy • Leading dual ion funnel technology for unparalleled sensitivity • Outstanding ETD/PTR applications for full PTM analysis • New SMART isolation and fragmentation • Unrivalled scan speed of 52,000 u/sec at a peak width < 0.5 u for resolution of doubly charged ions. APPLICATIONS: • High Sequence Coverage by CaptiveSpray™: The amaZon speed is equipped with the high-transmission, robust CaptiveSpray™ source for highest performance and reproducibility in bottom-up studies. • Market leading dynamic range: The amaZon speed ETD identifies proteins over at least 5 concentration levels in a single run. • Ultimate protein identification rates: Suvey view of a Hela cell digest (5µg) separated by a nano UHPLC and amaZon speed ETD equipped with a CaptiveSpray™ ion source. • Complete PTM characterization by Electron Transfer Dissociation (ETD): Many proteins execute their molecular function by reversible posttranslational modifications (PTMs) like e.g. phosphorylation. The identification and unambiguous localization of the modification site is of major importance to understand the biological context. ETD preserves the modification of the peptide backbone and therefore allows the clear assignment of the modification site. The amaZon speed is the unrivalled performance leader by its market proven ETD technology: most sensitive, robust and reliable.