CAPTIVESPRAY NANOBOOSTER - THE REVOLUTION IN PROTEOMICS IONIZATION

The Bruker CaptiveSpray principle: Stable and robust nanoflow LC/MS is still a challenge in proteomics analysis. The Bruker CaptiveSpray source is a revolutionary ion source with a patented design that provides easy operation just as simple as the normal flow electrospray. CaptiveSpray delivers nanospray sensitivity, resists plugging, and provides reproducible, uninterrupted flow for even the most complex proteomics samples. Plug-and-Play: The CaptiveSpray source mounts directly onto all current Bruker API mass spectrometers. The Ion Transfer Interface connects directly to the front of the capillary, eliminating the need for complex XYZ adjustable stages. Bruker's revolutionary CaptiveSpray source is now available with a smart dopant addition option—the nanoBooster: CaptiveSpray nanoBooster brings your MS to the next performance level and provides even higher flexibility. • Boosts nanoflow sensitivity • Pushes up ID rates • Enables Glycoanalysis • Supercharges capability. TECHNICAL DETAILS: • Ease of Use: CaptiveSpray provides a vortex gas that sweeps around the emitter spray tip to desolvate and focus the Taylor cone into the MS inlet capillary. The vacuum seal to the MS ion guide draws all of the sample ions into the MS increasing the efficiency of sample transfer from the spray tip into the mass spectrometer. The direct connection to the inlet capillary eliminates the need for any source adjustment making the CaptiveSpray source truly Plug-and-Play. • Smart Ionization Support by Dopant-enriched Gas Supply: The nanoBooster option allows the modification and vapor enrichment of gas that flows around the emitter. Dependent on the used dopant, either charge stripping or charge enhancement of peptides and proteins can be achieved during the ionization process. Charge enhancement is advantageous for the analysis of large biomolecules due to the reduction of the mass-to-charge (m/z) ratio. Furthermore, higher charge stages increase efficiency of electron transfer dissociation (ETD) MS/MS. APPLICATIONS: • Glycoanalysis: The enrichment of the nanoBooster sheath gas with acetonitrile results in improvement of glycopeptide analyzation. The charge state increase provides higher quality ETD spectra and enables highly improved glycopeptide characterization by assignment and identification of the glycosylation sites. • Supercharging in Gas Phase: The gas supply is software-controlled. The user can define the dopant enrichment and charge effect. CaptiveSpray nanoBooster provides the capability of post-column charging of peptides without the need to add buffers to the HPLC solvents and thus without influencing the chromatographic performance. • ID Rates: CaptiveSpray nanoBooster increases the ionization efficiency of peptides significantly, leading to a much higher sensitivity than even conventional nanospray. This results in a highly improved number of identified proteins in complex mixtures.